Introduction:
Custom Recombinant Antibody Development services offer a tailored approach to antibody production, revolutionizing the field of molecular biology and diagnostics. These services are designed to meet the specific needs of researchers and clinicians by generating highly specialized antibodies, typically monoclonal or polyclonal, using recombinant DNA technology. This approach allows for the precise manipulation of antibody properties, such as affinity, specificity, and isotype, to target a wide range of antigens, including proteins, peptides, and small molecules. Custom recombinant antibody services provide a cost-effective and rapid solution for researchers seeking high-quality antibodies for applications like immunohistochemistry, flow cytometry, and Western blotting. In this blog let’s explore the 3 important phases of custom recombinant antibody development, including gene synthesis and cloning, transfection and expression, and purification and delivery.
Phase 1: Gene Synthesis and Cloning
In the realm of custom recombinant antibody development, the initial phase is centered around gene synthesis and cloning, where the blueprint for the desired antibodies is meticulously crafted and transferred into host cells for production. It comprises three key sub-points:
Synthesize codon-optimized HC and LC: This step involves the meticulous design and synthesis of genes encoding the heavy chain (HC) and light chain (LC) of the desired antibody. Codon optimization ensures efficient protein expression in the chosen host cells.
Subclone in a proprietary expression vector: The synthesized genes are subcloned into a specialized expression vector, which serves as a vehicle for introducing these genes into the host cells. This vector contains elements that facilitate efficient antibody production.
Confirm by endpoint sequencing: To guarantee the accuracy of the gene constructs, endpoint sequencing is performed. This critical quality control step ensures that the cloned genes match the intended sequences.
Phase 2: Transfection and Expression
The second pivotal phase in custom recombinant antibody development encompasses transfection and expression, as it brings the engineered genes into action within host cells, driving the production of specific antibodies tailored for diverse research and clinical applications. This phase consists of the following steps:
Transfect a host cell line of interest (CHO or 293): The cloned gene constructs are introduced into the chosen host cell line, such as Chinese Hamster Ovary (CHO) cells or Human Embryonic Kidney (293) cells. Transfection is a key process, allowing the host cells to become antibody factories.
Culture under optimized protein-free conditions: The host cells are cultivated under carefully optimized conditions, often protein-free, to promote efficient antibody production. Precise culture conditions are essential to achieve high yields of the desired antibodies.
Harvest clear culture supernatants: After a successful expression process, clear culture supernatants rich in the desired antibodies are obtained. These supernatants contain the secreted antibodies and are prepared for the next phase.
Phase 3: Purification and Delivery
The final phase of custom recombinant antibody development focuses on purifying the antibodies and ensuring their quality and safety. It includes the following crucial steps:
Purification by protein G or A: Affinity chromatography, using Protein G or Protein A, is employed to isolate the antibodies from other components present in the culture supernatant. This purification step ensures high antibody purity.
Validate antibody by SDS-PAGE: The purified antibodies are rigorously validated using SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), confirming their integrity and purity.
Filter through a 0.22mm filter: Before delivery, the antibodies are filtered through a 0.22-micron filter to remove any remaining impurities and ensure sterility.
Clarify endotoxin level: Endotoxin levels are assessed to ensure the safety of the delivered antibodies. Low endotoxin levels are essential, especially for therapeutic applications.
Deliver antibodies to the customer: Finally, the purified and validated antibodies are delivered to the customer along with comprehensive documentation, including information on their characteristics and quality control data.
Custom Recombinant Antibody Development services by Abeomics
At Abeomics Inc., we are one of the leading authorities in custom recombinant antibody development services, providing a seamless and top-notch experience for our clients. What sets us apart is our unwavering commitment to precision, efficiency, and transparency. When you choose Abeomics, you can expect unparalleled expertise in the field. Our process begins with synthesizing the gene from the nucleotide sequence provided by the customer, ensuring that the recombinant protein production is of the highest quality. Additionally, our customer-centric approach means that we understand the urgency of your research, and we swiftly provide estimates for project costs and precise guidelines for project timelines. This allows you to plan your next steps with confidence, knowing that your custom recombinant antibody development project is in the hands of experts who prioritize your research goals and timelines. Abeomics is the trusted partner you need for successful antibody development services.
Conclusion:
In conclusion, custom recombinant antibody development services offer a tailored approach to obtaining high-quality antibodies, meeting diverse research and clinical needs. Understanding the workflow, from gene synthesis and cloning to transfection and expression, and purification and delivery, ensures the production of antibodies that excel in specificity and functionality. Collaborating with experienced service providers guarantees success in your research or applications.
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